PROCESS FOR THE ENZYMATIC PREPARATION OF BASIC FIBROBLAST GROWTH FACTOR

摘要

Novel prepn. of a basic fibroplast growth factor (bFGF) comprises (a) forming an adduct between heparin or heparan sulphate and a bFGF which has a 9-10 Leu-Pro bond, (b) treating the adduct with pepsin A or cathepsin D to cleave the bond and (c) releasing from the adduct the bFGF thus obtd. The bFGF may be of formula (I). NH2-(AA)x-Leu-Pro-Y-COOH (I). (AA = any amino acid residue, x = 0 or an integer; (AA)x may be eg. Ile Thr Thr, Thr Thr or Thr; y = the amino acid sequence of (11-155) bFGF.