| 摘要 |
<p>A method for detecting an amplified nucleic acid in a sample comprising the following steps: i) incorporating a ligand into each of nucleic strands by means of an amplification reaction using as polymerisation primers two primers, each having said ligand, in order to obtain a nucleic acid-ligand complex; ii) allow said nucleic acid-ligand complex to denaturate into single strands; iii) incubate under denaturing conditions said nucleic acid-ligand complex with a binding reagent specific for said ligand, wherein said binding reagent is adhered to a solid substrate, in order to obtain a nucleic acid-ligand-binding reagent complex which is adhered to a solid substrate; iv) reveal said nucleic acid-ligand-binding reagent complex. Also described is a kit to detect an amplified nucleic acid according to the method of the invention.</p> |
