| 摘要 |
The present invention provides a minimal essential medium for maintaining neural cell or tissue viability in an environment containing ambient levels of CO2. The medium contains less than about 2000 muM bicarbonate, a buffer having a pKa of from about 6.9 to about 7.7, wherein the medium is free of ferrous sulfate, glutamate and aspartate, from 0 to about 3000 muM CaCl2, from about 0.05 to about 0.8 muM Fe(NO3)3, from about 2500 to about 10000 muM KCl, from 0 to about 4000 muM MgCl2, from about 74000 to about 103000 muM NaCl, from about 400 to about 2000 muM NaHCO3, from about 250 to about 4000 muM NaH2PO4, from about 0.2 to about 2 muM ZnSO4, from about 2500 to about 50000 muM glucose and from about 23 to about 500 muM sodium pyruvate. The present invention also provides a process of extending neural cell or tissue viability in an atmosphere having ambient levels of carbon dioxide whereby the neural cells or tissue are placed in such a medium. Preferably, the medium is supplemented with a growth-promoting medium that contains effective amounts of hormones, essential fatty acids and anti-oxidants for neural cells. |
