发明名称 |
Kit for amplification and detection of nucleic acid of cytomegalovirus (cmv) utilizing a nucleic acid sequence (ss2.7) |
摘要 |
<p>The present invention relates to two primers for amplifying a cytomegalovirus (CMV) nucleic acid suitable for a nucleic acid sequence-based amplification (NASBA) using a DNA-dependent RNA polymerase, a first primer containing a promoter sequence and a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:1; a second primer containing a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:2, NO:3 or NO:4; a detecting probe and/or a capturing probe containing a nucleic acid sequence consisting of at least continuous fifteen bases selected from the nucleic acid sequence(s) of SEQ ID NO:5, NO:6 and/or NO:7 wherein said sequence is modified if necessary; a reagent kit for detecting a CMV containing the above-mentioned primers and probes; and a nucleic acid sequence-based amplification (NASBA) using said primers. The advantages of the present invention are that CMV can be detected in an easy, rapid and specific manner with a high sensitivity.</p> |
申请公布号 |
EP0803578(A2) |
申请公布日期 |
1997.10.29 |
申请号 |
EP19970106495 |
申请日期 |
1997.04.18 |
申请人 |
TOYO BOSEKI KABUSHIKI KAISHA |
发明人 |
KONDO, MOTOHIRO;AONO, TOSHIYA;SEGAWA, MASAYA;YAMANISHI, KOICHI;KONDO, KAZUHIRO;TAYA, KEIKO |
分类号 |
C12N15/09;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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