An in vitro assay method permits the identification of a test substance which inhibits the mutual association of a pair of proteins. The method includes the steps of providing a pair of proteins capable of mutual association, one of said proteins bearing a covalently linked fluorophore; preparing a mixture containing the two proteins and at least one test substance; irradiating the mixture with polarized light of a suitable wavelength permitting excitation of the fluorophore as indicated by emission of polarized light; measuring the degree of polarization of the emission, and determining the effect of the presence or concentration of the test substance in decreasing the observed emission polarization of a mixture of the two proteins alone. Inhibitory activity of in the test substance correlates with decreased depolarization values.
申请公布号
WO9739326(A2)
申请公布日期
1997.10.23
申请号
WO1997US06746
申请日期
1997.04.18
申请人
ARIAD PHARMACEUTICALS, INC.;LYNCH, BERKLEY, A.;MACNEIL, IAN, A.;ZOLLER, MARK, J.
发明人
LYNCH, BERKLEY, A.;MACNEIL, IAN, A.;ZOLLER, MARK, J.