发明名称 |
NOVEL REAGENT, METHOD, AND KIT FOR THE QUANTITATION OF OXIDATION-REDUCTION PHENOMENA IN PROTEINS AND PEPTIDES |
摘要 |
A first embodiment of the method is for analyzing the amount of methionine sulfoxide in a protein sample and includes the steps of contacting a protein solution with methionine sulfoxide reductase in the presence of a reducing reagent bearing a covalently-linked reporter tag, whereby the reducing reagent is oxidized. The oxidized reducing reagent formed, which is proportion to the amount of methionine sulfoxide in the sample, is then quantified. A second embodiment of the method is for analyzing the amount of disulfide linkages in a polypeptide or protein sample. It proceeds in the same fashion as above, but in the absence of any enzyme. A novel fluorescently-labeled reducing agent, and kits to practice the method are also disclosed. |
申请公布号 |
WO9739141(A1) |
申请公布日期 |
1997.10.23 |
申请号 |
WO1997US06152 |
申请日期 |
1997.04.14 |
申请人 |
PROMEGA CORPORATION |
发明人 |
SHULTZ, JOHN, W.;SELMAN, SUSANNE;SIMPSON, DANIEL, J. |
分类号 |
C07D271/12;C07D413/12;C12Q1/26;G01N31/22;(IPC1-7):C12Q1/26 |
主分类号 |
C07D271/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|