发明名称 |
Quantitative detection of specific nucleic acid sequences using lambdoid bacteriophages linked by oligonucleotides to solid support |
摘要 |
The present invention provided compositions, methods and kits for detection and quantitation of pathogenic organisms. The composition of the invention is an oligonucleotide probe comprising a bacteriophage covalently linked to one site on an oligonucleotide probe complementary to a conserved region of a pathogenic organism. At a second site, the oligonucleotide probe is linked to a matrix. The oligonucleotide probe contains a region complementary to one strand of a restriciton endonuclease recognition site or an oligoribonucleotide moiety. The number of pathogenic organisms present in a biological fluid sample may be quantitated in accordance with the method of the invention by combining the composition of the invention with the sample, allowing hybridization to occur. Hybridization generates a DNA-RNA hybrid, and by adding the appropriate nucleolytic enzyme capable of cleaving DNA-RNA hybrids; bacteriophage will be released for measurement. The kit of the invention provides components which allow the method of the invention to be performed.
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申请公布号 |
US5679510(A) |
申请公布日期 |
1997.10.21 |
申请号 |
US19950368870 |
申请日期 |
1995.01.05 |
申请人 |
HYBRIDON, INC. |
发明人 |
RAY, BRYAN L.;LIN, EDMUND C. C. |
分类号 |
C12N15/09;A61K47/48;A61K51/08;C07H21/04;C07K14/005;C07K14/01;C12N7/00;C12P21/02;C12Q1/02;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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