| 摘要 |
Highly efficient microcolumns suitable for use in chromatography, especially size excusion chromatography, are presented along with the method for making them. The method involves first obtaining a microcolumn having a first end and a second end, said microcolumn having an inner diameter of less than 1 mm. Then a slurry is prepared containing a packing material and a liquid solvent, said slurry having a concentration of more than 10 milliliters of solvent per gram of packing material. A retaining means is placed at the second end of the microcolumn, said retaining means being effective for retaining the packing material while allowing the liquid solvent to pass through the retaining means. The slurry is then added to the first end of the microcolumn at a constant pressure, said pressure being less than about 680 atmospheres. After a length of column suitable for use in chromatography has been filled, the addition of the slurry is stopped. The column is allowed to slowly decompress by allowing the solvent to exit the second end of the column.
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