Transformation of monocot cells

摘要

PCT No. PCT/EP93/00905 Sec. 371 Date Mar. 15, 1995 Sec. 102(e) Date Mar. 15, 1995 PCT Filed Apr. 14, 1993 PCT Pub. No. WO93/21335 PCT Pub. Date Oct. 28, 1993A process for genetically transforming the nuclear genome of a cell of a rice plant, comprising the steps of: providing without any enzymatic pretreatment or mechanical cell wall removal a culture of aggregated suspension cells which are preplasmolyzed in a hypertonic buffer, transforming said cells by electroporating and optionally regenerating a transformed plant.