| 摘要 |
A method of immediately discriminating bacteria which comprises mixing bacteria sampled from a specimen into a staining solution prepared by mixing physiological saline with a fluorescent dye comprising fluoresceine or a derivative thereof and another fluorescent dye comprising propidium iodide each in a required concentration and further with a staining accelerator in a given concentration, heating the resultant mixture to a required temperature to stain live and dead bacteria by causing the fluorescent dyes to penetrate into and disperse in the cells of the bacteria selectively, mixing the resultant mixture with an efflux inhibitor in a required concentration to prepare a stained bacteria solution, dropping this solution onto a light-transmitting plate which has been rendered light-scattering, irradiating the solution from under the plate with an exciting light having a central wavelength of 488 nm, and observing the fluorescent light emitted by the absorption of the exciting light, the configuration and size of the fluorescent emission, and the number of fluorescent spots through a magnifier from above, thereby determining the difference between live and dead bacteria, the type of bacteria, and the bacterial count; an apparatus for immediately discriminating bacteria which is provided with a source of an exciting light having a central wavelength of 488 nm in the lower part of a casing whose inside is kept in a state of darkroom, a magnifier above on the optical axis of the exciting light, and a light-transmitting plate which has been rendered light-scattering in the intermediate part; and another apparatus for immediately discriminating bacteria which is further provided with a band-pass filter for selecting the wavelengths of 520 nm and 625 nm above the magnifier and a video camera on the upper end of the filter, thereby converting the images which have passed the magnifier into electric signals and synthesizing the signals with a computer for image processing.
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