发明名称 |
In vitro differentiation of CD34+ progenitor cells into T lymphocytes |
摘要 |
The invention involves a method for the in vitro T cell production. A monolayer of non-human primate thymic stromal cells are cocultured in vitro with primate hematopoietic T cell progenitor cells. This results in the differentiation and growth of mature T cells. The T cells may be isolated at any sequential stage of differentiation and further expanded by coculture with a mitogenic agent. The T cells also may be genetically altered at any stage of the process. The effect of agents on the growth and differentiation of T cells may be measured by comparing a coculture containing the agent with a control coculture and comparing the differentiation or growth of the T cells progenitor cells in the test culture with the control culture. Kits and novel populations of T cells are provided.
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申请公布号 |
US5677139(A) |
申请公布日期 |
1997.10.14 |
申请号 |
US19950426782 |
申请日期 |
1995.04.21 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
JOHNSON, R. PAUL;ROSENZWEIG, MICHAEL;SCADDEN, DAVID T. |
分类号 |
C12N5/02;C12N5/0783;G01N33/50;(IPC1-7):C12Q1/02 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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