摘要 |
PROBLEM TO BE SOLVED: To obtain a new strain 92-KU-137-4 of Bacillus thuringiensis (Life technology industrial technical institute stipulation number FERM P-15230), having an insecticidal activity against Anopheles sinensis and Culex pipiens molestus and useful as an insecticide for Diptera noxious insects. SOLUTION: Tihs is a new strain 92-KU-137-4 of Bacillus thuringiensis (Life technology industrial technical institute, number FERM P-15230), and its cultured product is useful as an effective component of an insecticide having an insecticidal activity against Diptera noxious insect, such as Anopheles sinensis and Culex pipiens molestus. This new strain is obtained by weighing out dusts at a silk raising farmhouse is Kumamoto prefecture in a test tube holding sterilized water and efficiently suspending it followed by heat treatment under standing in a water bath at 70 deg.C for 10min., then spreading it on the ordinary agar medium after dilution, and incubating at 28 deg.C for 3-5 days. The colony obtained is observed by a phase contrast microscope and the strain containing white spores and black crystalline protein in same cells is selected out. |