Method for quantifying substrate using an enzyme electrode

摘要

A method for quantifying a substrate having improved measurement accuracy and measurement sensitivity is disclosed: a constant voltage is applied to an electrode system (27), between a working electrode and a counter electrode, via a resistor (20) connected in series with the electrode system after placing a sample solution containing the substrate on the electrode system, so as to oxidize an electron acceptor, which has been reduced by an enzyme reaction of the substrate, thereby determining a concentration of the substrate included in the sample solution based on an oxidizing current flowing in the course of oxidation.