发明名称 |
Eukaryotic cells comprising a DNA sequence encoding a bacterial-viral chimeric transactivator protein |
摘要 |
The present invention relates to novel chimeric transactivating proteins comprising a functional portion of a DNA binding protein and a functional portion of a transcriptional activator protein. The chimeric transactivating proteins of the invention offer a variety of advantages, including the specific activation of expression of genes engineered to comprise transactivator responsive elements, thereby achieving exceptionally high levels of gene expression. Furthermore, in various embodiments of the invention, the transactivator proteins may be used to increase expression of some genes while repressing the expression of others, thus permitting a greater degree of control of gene expression patterns than other currently available systems. In preferred embodiments of the invention, the function of the chimeric transactivator proteins may be induced, for example, by chemical agents (e.g. IPTG) or changes in temperature. In a preferred specific embodiment of the invention, the ability to switch transactivator function on and off may be utilized in the context of transgenic animals and to develop cell lines capable of differentiation.
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申请公布号 |
US5674730(A) |
申请公布日期 |
1997.10.07 |
申请号 |
US19930041297 |
申请日期 |
1993.03.30 |
申请人 |
THE TRUSTEES OF PRINCETON UNIVERSITY |
发明人 |
BAIM, STEVEN B.;LABOW, MARK A.;SHENK, THOMAS E.;LEVINE, ARNOLD J. |
分类号 |
C07K14/035;C12N15/85;(IPC1-7):C12N5/00;C12N15/00;C07H21/04 |
主分类号 |
C07K14/035 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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