发明名称 |
Continuous selective clonogenic expansion of relatively undifferentiated cells |
摘要 |
A system is provided for selective clonogenic expansion of relatively undifferentiated cells, including (a) a tube containing a plurality of beads of a size which permits a plurality of the undifferentiated cells to grow thereon, the beads bearing on their surfaces a selective binding molecule which binds to a surface antigen present on the relatively undifferentiated cells, wherein the antigen is not present on the surfaces of the relatively differentiated cells; (b) means for continuously providing nutrients to the relatively undifferentiated cells growing on the beads, wherein the nutrients are delivered via a fluid which flows through the tube and past the beads; and (c) means for continuously harvesting the relatively undifferentiated cells downstream of the beads.
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申请公布号 |
US5674750(A) |
申请公布日期 |
1997.10.07 |
申请号 |
US19950446165 |
申请日期 |
1995.05.19 |
申请人 |
T. BREEDERS |
发明人 |
KRAUS, MOREY;FRIBERG, JILL |
分类号 |
C12N11/14;A01N1/02;A01N63/00;A01N65/00;A61K45/00;C07K16/28;C12M3/00;C12N5/00;C12N5/02;C12N5/0789;(IPC1-7):C12N5/02;C12N5/08 |
主分类号 |
C12N11/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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