| 摘要 |
<p>Fine magnetic particles containing useful proteins bound thereto, which comprise fine magnetic particles formed in the cells of a magnetic bacterium and a hybrid protein bound to the organic membranes covering the particles, the hybrid protein being one composed of a membrane protein inherently bound to the organic membranes and one or more useful proteins bound thereto biologically by, for example, fusion. The biologically immobilized proteins do not undergo activity reduction. Enzymes, antibodies and other useful proteins can be obtained in a state immobilized onto fine magnetic particles merely by culturing a transformed magnetic bacterium and separating the fine magnetic particles formed in the bacterial cells. When a functional protein is thus immobilized, the function of such a protein can be exhibited efficiently at a desired location because the protein can be controlled magnetically. As it is possible to genetically control the site of protein expression, it becomes possible to conduct a highly sensitive detection when a binding protein such as protein A and a marker protein such as a photoprotein are expressed in proximity to each other on the magnetic particles.</p> |
