| 摘要 |
<p>Procedure for the analysis of the cell cycle of cell subpopulations present in heterogeneous cell samples. Consists of: sequential incubation of the sample with: 1) a pool of monoclonal antibodies conjugated with the same fluorochrome, and 2) a fluorochrome that specifically binds to nucleic acids; the measurement by flow cytometry of the fluorescence emissions; the specific determination of the cell cycle distribution of subpopulations of cells present in the sample identified using a multiparametric analysis which allows the calculation, for both cell groups, of the proportion of cells in the G0/G1, S and G2/Mitosis cell cycle phases.</p> |
