发明名称 |
Treatment of paraffin embedded tissue for gene analysis |
摘要 |
Rapid and easy preparation of a sample for a gene analysis or high-purity nucleic acid suitable for gene amplification especially, for example, by the PCR method is made possible by a process for treating a paraffin-embedded tissue sample to be used for a gene analysis, which comprises heating an aqueous suspension containing a surfactant having a protein-denaturational action and a deparaffinized tissue sample obtained from a paraffin-embedded tissue sample at 60 DEG C. or higher, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, mixing the resulting reaction solution with a solution containing an organic compound having a protein-denaturational action other than the aforesaid surfactant, and precipitating nucleic acid from the resulting reaction solution by addition of an alcohol.
|
申请公布号 |
US5672696(A) |
申请公布日期 |
1997.09.30 |
申请号 |
US19950498775 |
申请日期 |
1995.07.05 |
申请人 |
WAKO PURE CHEMICAL INDUSTRIES, LTD. |
发明人 |
WANG, LU;HIRAYASU, KAZUNARI |
分类号 |
C12Q1/68;(IPC1-7):C07H1/06;C07H1/08 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|