CHROMATOGRAPHIC IMMUNOASSAY DEVICE AND METHOD UTILIZING PARTICLE VALENCY FOR QUANTITATION

摘要

A method and device are provided for the semi-quantitative and quantitative determination of an analyte in a sample. A non-competitive trap which can bind unreacted labelled receptor to analyte but has virtually no binding capabilities to receptor in the presence of analyte is used. Labelled receptor: analyte complex is trapped in a second trap. The relative amounts of unbound receptor in the non-competitive trap versus the amount of receptor: analyte complex in the second trap is a measure of the amount of analyte in the sample.