发明名称 |
OSCILLATION APPARATUS AND METHODS FOR MULTI-ANALYTE HOMOGENEOUS FLUORO-IMMUNOASSAYS |
摘要 |
An apparatus and method for rapidly analyzing samples for analytes of interest by an homogeneous immunofluorescence assay. The apparatus includes a sample test cartridge having a high control sample section, a low control sample section, and at least one test sample section. Each of these sections contain at least one pre-loaded reagent housed in a well within the the cartridge wherein the low control sample section contains a known low amount of an analyte of interest and the high control sample section contains a known high amount of an analyte of interest. The cartridge includes a biosensor comprising a planar waveguide having first and second parallel plane surfaces and an edge extending between them, the edge having a receiving region for receiving a light beam. Each of the high control sample section, the low control sample section, and the test sample control sections have a well which includes a waveguide surface, wherein the contents of each section contacts capture molecules immobilized on the waveguide surface. The capture molecules are configured to specifically bind a chosen analyte and fluoresce when interacting with light passing through the waveguide surface. The concentration of said analyte of interest in said sample fluid is determined by a comparison of intensities of fluorescence of between said capture molecule areas of said sample capture molecule well, said low control capture molecule well, and said high control capture molecule well.
|
申请公布号 |
WO9735203(A1) |
申请公布日期 |
1997.09.25 |
申请号 |
WO1997US04378 |
申请日期 |
1997.03.19 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION;HERRON, JAMES, N.;CHRISTENSEN, DOUGLAS, A.;MILES, SCOTT, D. |
发明人 |
HERRON, JAMES, N.;CHRISTENSEN, DOUGLAS, A.;MILES, SCOTT, D. |
分类号 |
G01N33/543;(IPC1-7):G01N33/552 |
主分类号 |
G01N33/543 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|