发明名称 |
TARGET NUCLEIC ACID SEQUENCE AMPLIFICATION |
摘要 |
A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of those sites, and extensions from those nicks, thereby displacing any existing polynucleotides. Further provided is a method for detecting polynucleotides using the method of the invention. A kit combining the components commonly used in practicing the method of the invention is also provided.
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申请公布号 |
WO9735026(A1) |
申请公布日期 |
1997.09.25 |
申请号 |
WO1997US04170 |
申请日期 |
1997.03.17 |
申请人 |
MOLECULAR BIOLOGY RESOURCES, INC. |
发明人 |
WICK, JAMES, F.;MUELLER, REINHOLD;BLASSAK, MICHELE, L.;WILKOSZ, RICHARD, K. |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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