| 摘要 |
PCT No. PCT/GB94/02259 Sec. 371 Date Apr. 8, 1996 Sec. 102(e) Date Apr. 8, 1996 PCT Filed Oct. 14, 1994 PCT Pub. No. WO95/10604 PCT Pub. Date Apr. 20, 1995An enzyme is provided which has acylase activity capable of hydrolyzing N-acetyl-(R,S)-pipecolic acid stereoselectively to give (S)-pipecolic acid, wherein this activity is greater than that on N-acetyl-S-proline, at pH 7.5, 25 DEG C., and substrate concentration 20 g/l, in 75 mM Tris buffer. The enzyme is obtainable from the species of Alcaligenes denitrificans deposited as NCIMB 40587. A microorganism having therein the enzyme also is provided, as is a process for preparing (S)-pipecolic acid comprising contacting a mixture of enantiomers of an N-acyl-pipe colic acid with the enzyme or microorganism. |
