发明名称 |
Recombinant fowlpox virus |
摘要 |
An improved method is described to prepare recombinant fowlpox virus for the expression of proteins or for use as a vaccine. The new method uses for the insertion of foreign DNA an intergenic region which is located between the FPV thymidine kinase (tk)gene and the 3'-open reading frame. Said intergenic region is enlarged to comprise one or more unique restriction sites, thereby allowing insertion of foreign DNA in such a way that the FPV tk-gene remains intact and codes for the entire thymidine kinase. New strong poxvirus promoters are presented and new FPV host virus strains carrying a vaccinia virus thymidine kinase gene and an E. coli lacZ gene as a novel non-essential site. The novel fowlpox virus host strains allow the use of any insertion plasmid carrying vaccinia virus tk-gene flanking regions.
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申请公布号 |
US5670367(A) |
申请公布日期 |
1997.09.23 |
申请号 |
US19940232463 |
申请日期 |
1994.04.22 |
申请人 |
IMMUNO AKTIENGESELLSCHAFT |
发明人 |
DORNER, FRIEDRICH;SCHEIFLINGER, FRIEDRICH;FALKNER, FALKO GUNTER |
分类号 |
A61K39/275;A61K39/00;A61K39/395;A61K48/00;A61P31/12;C12N5/10;C12N7/00;C12N15/09;C12N15/39;C12N15/85;C12N15/86;C12N15/863;C12P21/02;(IPC1-7):C12N15/86;C07H21/045 |
主分类号 |
A61K39/275 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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