发明名称 |
Method and apparatus for three-dimensional microscopy with enhanced depth resolution |
摘要 |
A method and apparatus for three dimensional optical microscopy is disclosed which employs dual opposing objective lenses about a sample and extended incoherent illumination to provide enhanced depth or Z direction resolution. In a first embodiment, observed light from both objective lenses are brought into coincidence on an image detector and caused to interfere thereon by optical path length adjustment. In a second embodiment, illuminating light from an extended incoherent light source is detected to the sample though both objective lenses and caused to interfere with a section of the sample by adjusting optical path lengths. Observed light from one objective lens is then recorded. In a third embodiment, which combines the first two embodiments, illuminating light from an extended incoherent light source is detected to the sample through both objective lenses and caused to interfere with a section of the sample by adjusting optical path lengths. The observed light from both lenses is caused to interfere on the image detector by the same optical path length adjustment.
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申请公布号 |
US5671085(A) |
申请公布日期 |
1997.09.23 |
申请号 |
US19950384111 |
申请日期 |
1995.02.03 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
GUSTAFSSON, MATS G.L.;SEDAT, JOHN W.;AGARD, DAVID A. |
分类号 |
G01N21/27;G02B21/00;G02B21/06;G02B21/22;(IPC1-7):G02B21/06 |
主分类号 |
G01N21/27 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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