发明名称 |
Alteration of sequence of a deleterious target molecule by ribozyme catalyzed trans-splicing |
摘要 |
Method for splicing a target nucleic acid molecule with a separate nucleic acid molecule. Such splicing generally causes production of a chimeric protein with advantageous features over that protein naturally produced from the target nucleic acid prior to splicing. The method includes contacting the target nucleic acid molecule with a catalytic nucleic acid molecule including the separate nucleic acid molecule. Such contacting is performed under conditions in which at least a portion of the separate nucleic acid molecule is spliced with at least a portion of the target nucleic acid molecule to form a chimeric nucleic acid molecule. In this method, the catalytic nucleic molecule is chosen so that it is not naturally associated with the separate nucleic acid molecule.
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申请公布号 |
US5667969(A) |
申请公布日期 |
1997.09.16 |
申请号 |
US19930152450 |
申请日期 |
1993.11.12 |
申请人 |
UNIVERSITY RESEARCH CORPORATION |
发明人 |
SULLENGER, BRUCE A.;CECH, THOMAS R. |
分类号 |
C12N15/00;A61K38/00;A61K48/00;C07H21/04;C12N9/00;C12N15/113;(IPC1-7):C12Q1/70;C12Q1/68;C12P19/34 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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