摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new gene coding for a polypeptide having a specific amino acid sequence, to be induced in astrocytes when exposed to a hypoxic condition such as cerebral ischemia-reperfusion followed by being exposed to oxygen again, thus useful for preventing/treating cerebral ischemic diseases. SOLUTION: This gene is a new cerebral ischemia-related gene containing a base sequence coding for a polypeptide composed of a amino acid sequence of the formula. This gene is expressed and induced in astrocytes when exposed to a hypoxic condition such as cerebral ischemia-reperfusion followed by being exposed to oxygen again, therefore being useful for preventing/treating cerebral ischemic diseases of producing new polypeptides. This gene is obtained by the following process: a cerebral hemisphere is extracted from a SD-strain rat just after born and then put to enzymegenation to isolate cells which, in turn, are cultured in a medium spiked with cytosine arabinofuranoside and astrocytes are separated followed by isolating mRNA by conventional method, and the mRNA is subjected to PCR using a reverse trnascriptase and a primer to effect cloning. |