| 摘要 |
<p>A laminated membrane containing an enzyme is prepared for use with an electrode of a polarographic cell to assay for an analyte such as glucose or lactate. The membrane (28) has an outer or support layer (30), an inner or barrier layer (32) and an enzyme layer (34) between the outer and inner layers. The outer layer may be made of polycarbonate, regenerated cellulose or polyurethane polymer, and has pore sizes greater than about 200 Angstroms and preferably about 380-750 Angstroms, a porosity of 0.005 - 0.2 % and a thickness of about 5 to 7 microns. The inner layer may be made of a cellulose acetate/cellulose acetate butyrate polymer and has a thickness of less than 2 microns. The enzyme layer is typically cross-linked in situ between the outer and inner layers by the use of glutaraldehyde. The outer layer can have a multi-layered structure.</p> |
