| 摘要 |
<p>The present invention provides a detection system suitable for use in a method of detecting the presence or absence of a predetermined nucleic acid sequence in a sample of nucleic acid. The system comprises a first oligonucleotide primer capable of annealing with a first strand of nucleic acid containing said predetermined nucleic acid sequence, such that a 3' end region of the first primer anneals with a first portion of said predetermined nucleic acid sequence; a second oligonucleotide primer having a 3' end region capable of annealing with a second strand of nucleic acid complementary to said first strand of nucleic acid such that a 3' end region of the second primer anneals with a portion of said second strand which portion is complementary to a second portion of said predetermined nucleic acid sequence, which second portion is spaced from end 5' of said first portion. One of the first and the second primers is labelled and the other is anchored to a substrate. Upon subjecting a sample of nucleic acid to an amplification reaction in the presence of said first and second oligonucleotide primers the presence of said predetermined nucleic acid sequence is indicated by the production of an amplified labelled nucleic acid product captively retained on said substrate.</p> |
