A METHOD FOR THE AMPLIFICATION AND DETECTION OF A NUCLEIC ACID FRAGMENT OF INTEREST

摘要

A method is provided for the replication and detection of a specific nucleic acid target using a detection probe. The probe is present throughout the amplification reaction but does not participate in the reaction in that it is not extended. The probe contains sequence complementary to the replicated nucleic acid analyte for capture of the analyte by hybridization. Additionally, the probe or analyte contains at least one reactive ligand to permit immobilization or reporting of the probe/analyte hybrid.