发明名称 |
METHOD FOR CLEAVING CHIMERIC PROTEIN USING PROCESSING ENZYME |
摘要 |
A chimeric protein is provided which comprises a target protein and has been designed so as to be easily cleaved by a processing enzyme, permitting a target protein to be efficiently recovered. The chimeric protein is represented by the following formula: A-L-B wherein A represents a protective peptide; B represents a target peptide; and L represents a linker peptide having the sequence X1-X2-(Pro, Lys, or Arg)-Arg, wherein X1 and X2 represent any amino acid, in its C-terminal region and a domain rich in His in its N-terminal region. |
申请公布号 |
CA2198966(A1) |
申请公布日期 |
1997.09.04 |
申请号 |
CA19972198966 |
申请日期 |
1997.03.03 |
申请人 |
SUNTORY LIMITED |
发明人 |
SUZUKI, YUJI;MASUDA, TOYOFUMI;MAGOTA, KOJI |
分类号 |
C07K14/585;C07K14/635;C12N1/21;C12N9/38;C12N15/62;(IPC1-7):C12N15/62;C07K19/00 |
主分类号 |
C07K14/585 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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