| 摘要 |
<p>A method and apparatus for determining enzyme activities by supplying a substrate solution and an enzyme solution to a reaction vessel to which a total reflection absorption prism is connected. An assay solution (40) comprising a mixture of an enzyme solution and a substrate solution is brought to a constant temperature in a reaction vessel (26) by a thermostat (23) and stirred by an agitator (21). An infrared ray (36) sent out of an infrared ray source (3) enters an interface between a total reflection absorption prism (28) in contact with the assay solution (40) and the solution (40) from the side of the prism (28), and is totally reflected thereupon. The spectrum of the totally reflected, outgoing transmitted infrared ray (37) is detected by an infrared ray detector (5), and the variation of the infrared ray absorption spectrum or absorbance is determined on the basis of what has been detected, whereby the enzyme reaction in the object solution (40) is assayed.</p> |
