ANTIANDROGENIC AGENTS AND METHODS OF IDENTIFICATION THEREOF

摘要

A novel assay for identifying compounds with antiandrogenic activity employing a hamster ductus deferens cell line (DDT1) ATCC CRL-1701 and ATCC CRL-12051 is disclosed, as well as antiandrogenic compounds identified from this assay, particularly the nonsteroidal compound N-(4-chlorophenyl)-(Z,Z)-2,3-bis(cyclopropylmethylene)cyclopentane carboxamide. This compound is an antiandrogen useful in the treatment and prevention of diseases of the prostate including prostatitis, benign prostatic hyperplasia (BPH) and prostatic carcinoma. The present invention also provides novel compositions employing compounds which exhibit antiandrogenic activity in the assay of the present invention, as well as those described in previously described publications, and their use in combination in methods of treatment for diseases of the prostate or in pharmaceutical compositions.