| 摘要 |
<p>A method for the selective engraftment of hematopoietic stem cells is provided for increasing the efficiency of hematopoietic stem cell engraftment in vivo, comprising: (a) administering to a mammal a population of stem cells, comprising transgenic stem cells, the genome of which has been augmented by a first preselected DNA segment, which encodes resistance to an agent, and which said first preselected DNA segment is operably linked to a promoter functional in stem cells, wherein the expression of the first preselected DNA segment in the transgenic stem cells is sufficient to impart resistance or tolerance to said transgenic cells to an amount of the agent which is toxic to nontransgenic stem cells, the genome of which has not been augmented by the first preselected DNA segment; and (b) administering said agent to said mammal in an amount and for a time so as to increase the engraftment of transgenic stem cells relative to the engraftment of nontransgenic stem cells.</p> |
