| 摘要 |
<p>PURPOSE:To obtain a culture capable of exhibiting especially powerful nematocidal activity, by inducing marigold in a special culture medium into calli and propagating the resultant calli in a culture medium containing auxin in a low concentration. CONSTITUTION:Tissual pieces collected from leaves, stems, roots buds, etc., of marigold are sterilized by a conventional method and induction of calli is then carried out in a callusing culture medium containing auxin or further cytokinin as a phytohormone. A culture medium containing the auxin in a concentration as low as 0.01-1mg/l and in the absence of cytokinin or in a concentration thereof as low as <=3mg/l is used as a culture medium for successively cultivating the marigold induced into the calli.</p> |
