Assay preparation containing capture and detection polynucleotides covalently bound to substrates with a heterobifunctional crosslinking agent

摘要

Heterofunctional crosslinking agents are used to produce a device for detecting a target polynucleotide. Synthetic single stranded capture and detector polynucleotides are covalently immobilized to plastic surfaces by novel heterofunctional reagents. The capture and detector polynucleotides are covalently attached through a synthetic region of amine-bearing nucleotides that interact with reactive moieties on the heterofunctional crosslinker. The regions of the capture and detector polynucleotides that can hybridize a diagnostically important target polynucleotide are protected from binding to a solid phase support by intrastrand pairing. This system provides monolayers of immobilized polynucleotides with precise orientation and unimpaired diagnostic coding regions.