| 摘要 |
PROBLEM TO BE SOLVED: To isolate the above enzyme, derived from a coryneform bacterium and capable of catalyzing a pentose phosphate cycle according to a gene recom bination technology. SOLUTION: This glucose 6-phosphate dehydrogenase has an amino acid sequence represented by the formula. The enzyme is obtained by expressing a DNA (hereinafter referred to as a zwf gene), obtained from a chromosome of a coryneform bacterium, isolated and determined from a Brevibacterium flavum ML-233 (FERM BP-1497) strain and capable of coding the glucose 6-phosphate dehydrogenase in a coryneform bacterium. When the coryneform bacterium is transformed with the zwf gene, a bacterium capable of highly producing the glucose 6-phosphate dehydrogenase is obtained. |
