| 摘要 |
<p>In accordance with the present invention, there are provided a cell culture apparatus (10) and methods for the replenishment of cell culture media in a cell culture container (12) without disturbing the cell culture media therein. The cell culture apparatus (10) is made up of a cell culture container (12) shaped to hold cell culture media; a tube (14) comprising a first portion (16) and a second portion (18), wherein said first portion (16) is within said cell culture container (12), and said second portion (18) is outside said cell culture container (12); a pH sensor (20) capable of operative communication with the cell culture media in which the cell culture is growing; a pump (22), wherein said pump (22) is in operative communication with said second portion (18) of said tube (14); a source (24) of fresh culture media in fluid communication with said second portion (18) of said tube (14); a receptacle (26) for waste cell culture media in fluid communication with said second portion (18) of said tube (14); and a control device (28), wherein said control device (28) is in operative communication with said pump (22) and said pH sensor (20), said control device (28) controlling said pump (22) in response to said pH sensor (20).</p> |
