发明名称 |
SOLID PHASE SYNTHESIS OF OLIGONUCLEOTIDE N3' P5' PHOSPHORAMIDATES |
摘要 |
The invention provides a method of synthesizing oligonucleotide N3'P5' phosphoramidates using an amine-exchange reaction of phosphoramidites in which a deprotected 3'-amino group of a solid phase supported oligonucleotide chain is exchanged for the amino portion of a 5'-phosphoramidite of an incoming monomer which has a protected 3'-amino group. The resulting internucleotide phosphoramidite linkage is then oxidized to form a stable protected phosphoramidate linkage. The method of the invention greatly improves product yields and reduces reagent usage over currently available methods for synthesizing the above class of compound.
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申请公布号 |
CA2245666(A1) |
申请公布日期 |
1997.08.28 |
申请号 |
CA19962245666 |
申请日期 |
1996.06.14 |
申请人 |
LYNX THERAPEUTICS, INC. |
发明人 |
GRYAZNOV, SERGEI M.;MCCURDY, SARAH N.;NELSON, JEFFREY S.;HIRSCHBEIN, BERNARD L.;SCHULTZ, RONALD G.;FEARON, KAREN L. |
分类号 |
C07H19/10;C07H19/20;C07H21/00;C07H23/00;(IPC1-7):C07H21/00 |
主分类号 |
C07H19/10 |
代理机构 |
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地址 |
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