Production and purification of retroviral particles using tentacle anion exchange
摘要
This invention is directed to a continuous process for producing viral particles by providing in a perfused growth medium a population of viable virally infected non-lytic cells, and removing medium containing said cells at a rate to maintain the steady-state log-phase growth of cells remaining in the perfused growth medium. The invention is also directed to a process for purifying retroviral particles by passing a solution comprising the retroviral particles and contaminants through an anion exchange resin, and eluting the retroviral particles from the resin. The preferred exchange resin is a tentacle anion exchange resin.
申请公布号
US5661023(A)
申请公布日期
1997.08.26
申请号
US19960613920
申请日期
1996.03.11
申请人
THE IMMUNE RESPONSE CORPORATION
发明人
HRINDA, MICHAEL E.;PRIOR, CHRISTOPHER P.;MITSCHELEN, JONATHAN J.;IRISH, THOMAS W.;WEBER, DAVID M.;GORE, RICHARD S.;HARTER, JAMES J.;BAY, PIERRE M.;TARR, GEORGE C.