| 摘要 |
<p>A system and method of detecting diseased cells in a large population of normal cells that uses flow cytometry and vibrational spectroscopy techniques. The system and method combine flow cytometry with a spectrometric system to detect inherent properties of each cell examined, inherent properties that not only discriminate between the presence or absence of disease but also the degree at which the cell is diseased. More specifically, the system and method combine the use of flow cytometry to separate cells of the sample population and Raman or resonance Raman spectroscopic techniques for detecting inherent differences in cells for the purpose of determining whether cells are diseased and level of disease in the cells. This is accomplished by forming a stream of cells by flow cytometry into the sampling region at which each cell is irradiated by an incident beam of a Raman laser. The scattered Raman spectrum from each cell is detected by a Raman spectroscopy. More specifically, a complete Raman spectrum or resonance Raman spectrum is detected for each cell flowing past the incident beam. This will provide information as to whether the specific cell in the beam of the incident light is normal or diseased, and the level of disease also may be determined on the details of the spectrum detected.</p> |
