| 摘要 |
<p>The invention relates to a population of protein molecules having a distribution of specific mutations in the amino acid sequence as compared to a parent protein, which population of protein molecules is obtainable by establishing the parental amino acid sequence of the protein to be mutated and deriving therefrom a parental nucleotide sequence encoding the amino acid sequence, optionally selecting sites within this parental amino acid sequence, mutation of which is more desirable or less desirable, designing overlapping oligonucleotides encoding parts of the protein and harbouring one or more codon changes as compared to the corresponding parental nucleotide sequence, composing of the overlapping oligonucleotides a population of mutated nucleotide sequences encoding mutated versions of the parent protein thus establishing a library, and expressing the mutated versions of the mutated nucleotide sequences to obtain a population of mutated proteins.</p> |
