发明名称 |
PROCESS FOR CHROMATOGRAPHIC SEPARATION OF PEPTIDES AND NUCLEIC ACID, AND NEW HIGH AFFINITY ION EXCHANGE MATRIX |
摘要 |
Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, beta -lactoglobulin 1 and beta -lactoglobulin 2 on the anion exchanger (I) is higher than the elution ionic strength required for a quaternary comparative ion exchanger. Chromatofocusing and separation on ECTEOLA-cellulose is not included. New anion exchangers which are according to structure (I) wherein R1-6 are hydrocarbon groups which can contain ether oxygen atoms, hydroxyl groups, and different types of amino groups. X is OH or alkoxy or amino. B is a ligand arm replacing a group R1-6 or a hydrogen in any of R1-6. If one of the groups R5 and R6 contains a nitrogen atom then one of the groups also contains a hydroxy or alkoxy group. ECTEOLA-cellulose and variants where both R5 and R6 are selected among methyl and ethyl are not included.
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申请公布号 |
WO9729825(A1) |
申请公布日期 |
1997.08.21 |
申请号 |
WO1997SE00237 |
申请日期 |
1997.02.14 |
申请人 |
PHARMACIA BIOTECH AB;BERGLUND, ROLF;BERGSTROEM, JAN;BELEW, MAKONNEN;SOEDERBERG, LENNART |
发明人 |
BERGLUND, ROLF;BERGSTROEM, JAN;BELEW, MAKONNEN;SOEDERBERG, LENNART |
分类号 |
B01D15/08;B01D15/36;B01J41/20;C07K1/18;(IPC1-7):B01D15/08;B01J41/06 |
主分类号 |
B01D15/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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