| 摘要 |
Method for assessment of carbohydrate-deficient transferrin (CDTF) in a transferrin (TF) contg. body fluid, comprises: (a) obtaining a TF contg. liq. sample of or derived from the fluid, (b) contacting the sample with a source of iron ions, (c) contacting the sample with an anionic ion exchange resin at a pH so as to cause CDTF to be retained by the resin, (d) contacting the resin with an eluant to release the CDTF into the eluate, (e) collecting a vol. of the eluate, free from tetra- and penta-sialo TF and (f) assessing the TF variant content in the vol. of eluate. Also claimed are: (1) a kit for a diagnostic assay for CDTF, comprising: (a') an iron ion contg. buffered incubation soln. having a pH of /- 6.2, (b') opt. a TF soln. of known concn., (c') an anionic ion exchange resin packed in a container having sample introduction and eluate removal ports, (d') opt. a flushing eluant having a pH and ionic strength insufficient to release TF from the resin, (e') a release eluant having a pH and ionic strength sufficient to release TF from the resin, (f') opt. a light transmitting eluate receiving vessel, (g') opt. an anti-TF antibody or fragment and (h') opt. an opacification enhancer; (2) a method for assaying for alcohol consumption, comprising assessing a blood derived body fluid sample for its content of a de-sialo TF, characterised in that the de-sialo TF comprises tri-sialo TF and opt. /- 1 of di-sialo, mono-sialo and a sialo TF; (3) a method for assaying for alcohol consumption, comprising: (a'') obtaining a first TF contg. liq. sample of or derived form a body fluid and contg. tetra- and penta-sialo TFs, (b'') obtaining from the first sample a second TF contg. liq. sample which is free form tetra- and penta-sialo TF and having a TF content of /- 1 mg/l and (c'') turbidimetrically assessing the TF content of the second sample. |
