| 摘要 |
<p>Procedure for the detection and quantification of diarrheic toxins from dinoflagellates (DSP (diarrheic shellfish poison)) based on phosphatase inhibition. The presence of diarrheic producing blooms (DSP (diarrheic shellfish poison)) red tides contaminates shellfish, which toxicity must be continuously monitored before marketing. The current technique is the bioassay, which essentially is the oral administration of an extract to mice. The results is considered to be positive if the mouse dies after an observation period of 12 hours. This method is slow, costly, erratic and of low specificity. The procedure detects and quantifies the toxins by measuring the inhibition of phosphatases, which are the enzymes upon which these toxins act. This inhibition of phosphatases is quantified on a microtiter plate, and the activity is revealed by checking the activity of the enzymes with a phosphatase substrate which, depending on the chemical characteristics, can be converted to a fluorescent, luminescent or colored product. This technique provides rapidity, low cost and reliability. The industrial and canning seafood sector will be the most favored.</p> |
