发明名称 |
Novel creatine amidinohydrolase, production thereof and use thereof |
摘要 |
A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine + H2O -> sarcosine + urea Optimum temperature: about 40 - 50 DEG C Optimum pH: pH about 8.0 - 9.0 Heat stability: not more than about 50 DEG C (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5 - 10.0 mM Molecular weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor. According to the present invention, a creatine amidinohydrolase having a smaller Km value than that of the conventionally known creatine amidinohydrolase can be produced in an industrially large amount, and can be used as a routine reagent for clinical tests for determining creatine and creatinine in biological samples. |
申请公布号 |
EP0790303(A1) |
申请公布日期 |
1997.08.20 |
申请号 |
EP19970102270 |
申请日期 |
1997.02.13 |
申请人 |
TOYO BOSEKI KABUSHIKI KAISHA |
发明人 |
SOGABE, ATSUSHI;HATTORI, TAKASHI;NISHIYA, YOSHIAKI;KAWAMURA, YOSHIHISA |
分类号 |
C12N15/09;C07H21/04;C12N1/21;C12N9/78;C12N15/00;C12N15/52;C12Q1/34;C12R1/05;C12R1/19;G01N33/52 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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