发明名称 |
Promotion of epithelial cell adhesion and hemidesmosome assembly by a laminin-like molecule |
摘要 |
A method for growing epithelial cells in vitro using soluble proteins secreted by 804G and NBT-II rat bladder carcinoma cells. These proteins stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The protein is purified from conditioned medium by immunoaffinity chromatography using a monoclonal antibody generated against the protein. The purification of these proteins may be facilitated by culturing the cells under low serum or serum free conditions.
|
申请公布号 |
US5658789(A) |
申请公布日期 |
1997.08.19 |
申请号 |
US19950445135 |
申请日期 |
1995.05.19 |
申请人 |
DESMOS, INC. |
发明人 |
QUARANTA, VITO;HORMIA, MARKETTA |
分类号 |
C12P21/02;A61K35/12;A61L27/22;A61L27/34;A61L27/38;A61L29/08;A61L31/00;A61L31/10;A61L33/00;C07K14/47;C07K14/78;C07K16/18;C12N5/00;C12N5/071;C12R1/91;(IPC1-7):C12N5/00;C12N5/08;C07K14/00 |
主分类号 |
C12P21/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|