摘要 |
A method of preparing an immunity memory cell suspension, wherein cells consisting of T-cells are separated from cells collected from an immunity sensitized individual immunized against a particular pathogen and are mixed with an antibody reactive to a histocompatibility antigen specific to a prescribed species of cells for producing antigen-antibody complexes by the antibody and those of the separated cells which have the histocompatibility antigen, whereupon a blood serum is added to the cells containing the antigen-antibody complexes for causing the complement in the blood serum to bind to the antigen-antibody complexes and thereby destroying the cells having the histocompatibility antigen and allowing the remaining cells to persist. To these persisting cells is then added an antibody reactive to a histocompatibility antigen specific to immunity memory cells and from the resultant cells are separated the immunity memory cells which have the histocompatibility antigen specific to immunity memory cells. Immunity memory cells may be cultured and subcultured successively through a plurality of generations to establish a continuous line of cells which originates in the immunity memory cells thus separated from the initial immunity sensitized individual. |