发明名称 |
System for automated performance of the polymerase chain reaction |
摘要 |
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
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申请公布号 |
US5656493(A) |
申请公布日期 |
1997.08.12 |
申请号 |
US19940199505 |
申请日期 |
1994.02.18 |
申请人 |
THE PERKIN-ELMER CORPORATION |
发明人 |
MULLIS, KARY B.;JOHNSON, LARRY;LEATH, RICHARD A.;WENNBERG, TIMOTHY J.;MEZEI, LOUIS M.;WIDUNAS, JOSEPH T. |
分类号 |
B01J19/00;B01L7/00;C12Q1/68;C40B40/06;C40B60/14;(IPC1-7):C12M3/02;C12M1/00;G01N21/00;C12N15/00 |
主分类号 |
B01J19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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