| 摘要 |
An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissue equivalents wherein ice seeding is performed by discharging a liquefied or chilled gas (60) from a nozzle (21) to the surface of a container (40) containing said biological sample in cryopreservative. Inside a freezing chamber (70), containers (40) are placed on racks (30) that are located proximal to a sprayrail (20) containing nozzles (21). Ice seeding is performed by discharge of liquefied or chilled gas (60) from the nozzles to the container surface at the solid-liquid equilibrium temperature of the cryopreservative. Localized cooling of the container surface by the discharge results in ice seed formation within the container (40).
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