发明名称 |
EXPRESSION OF GROUP B NEISSERIA MENINGITIDIS OUTER MEMBRANE (MB3) PROTEIN FROM YEAST AND VACCINES |
摘要 |
The present invention relates, in general, to a method for obtaining the outer membrane protein meningococcal group B porin proteins, in particular MB3, and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in yeast. The invention also relates to a method of high level expression of the above-mentioned proteins wherein the rate of protein expression is enhanced by substituting a nucleotide sequence for the 5' region of the gene encoding said protein wherein the sequence has been optimized for yeast codon usage. The invention also relates to a vaccine comprising group A meningococcal polysaccharide (GAMP), group B meningococcal polysaccharide (GBMP) and group C meningococcal polysaccharide (GCMP) antigens, together with a pharmaceutically acceptable carrier. The invention also relates to a method of inducing an immune response in a mammal, comprising administering the abovementioned vaccine to a mammal in an amount sufficient to induce an immune response.
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申请公布号 |
CA2244989(A1) |
申请公布日期 |
1997.08.07 |
申请号 |
CA19972244989 |
申请日期 |
1997.01.31 |
申请人 |
NORTH AMERICAN VACCINE, INC. |
发明人 |
TAI, JOSEPH Y.;MINETTI, CONCEICAO A. S. A.;DONETS, MIKHAIL;MICHON, FRANCIS;POLVINO-BODNAR, MARYELLEN;WANG, MING-DER;LIANG, SHU-MEI |
分类号 |
A61K39/095;A61K39/385;C07K14/22;C07K19/00;C12N1/19;C12N15/31;(IPC1-7):C12N15/31 |
主分类号 |
A61K39/095 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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